In UC 0093, the Federation has recovered from its defeat and has created a new anti-colonial special forces unit to deal with rebel forces: Londo Bell. Elsewhere in space, Char Aznable reappears out of self-imposed hiding with a declaration that he now commands his own Neo-Zeon movement, and intends to force the emigration of Earth's inhabitants to space by bringing about an apocalypse.
Seven years after the defeat of the spiral king, Simon and the Dai-Gurren brigade must set out to the vastness of space to defeat a new threat and save the universe.
Simon and Kamina live in an underground city monitored by the village chief. When Simon stumbles upon an artifact and beastmen invade from the surface, Simon and Kamina rebel against them.
In the aftermath of the Fourth Impact, stranded without their Evangelions, Shinji, Asuka and Rei find refuge in one of the rare pockets of humanity that still exist on the ruined planet Earth. There, each lives a life far different from their days as an Evangelion pilot. However, the danger to the world is far from over. A new impact is looming on the horizon—one that will prove to be the true end of Evangelion.